BACKGROUND: In the past decades, many researcher aims to repair irreversible impaired hearts. Cardiac tissue engineering has been proposed as appropriate method to resolve this problem. Bioengineered cardiac grafts are created by combining functional cells with a degradable scaffold as a temporary extracellular matrix. In this study, biodegradable PLCL scaffold seeded with MSC may hold potential for the repair of damaged heart tissue. METHODS: Myocardial infarction was induced by cryoinjury method. Mesenchymal stem cells were isolated from bone marrow aspirates of isogenic inbred Lewis rat and expanded in vitro. MSCs were labeled with CM-DiI and seeded on biodegradable poly (lactide-co-epsilon-caprolactone) scaffold. At ten days after myocardial infarction, saline (Group Ⅰ, n=8); scaffold (Group Ⅱ, n=8); MSCs (Group Ⅲ, n=8); MSCs seeded scaffold (Group Ⅳ, n=8) was administrated to rats. Four weeks after implantation, echocardiography was performed to evaluate cardiac function. After then, hearts were harvest for histological analysis. RESULTS: LV ejection fraction in Group Ⅳ was increased 22.5% compared to Group Ⅰ. LV diastolic dimension in Group Ⅳwas attenuated 23% compared to Group Ⅰ. Infarct size in Group Ⅳ (31.2±2.6%) was significantly attenuated compared with other three groups (38.8±4.3%; 37.8±3.2%; 36.2±3.6%). CONCLUSIONS: MSCs with Biodegradable PLCL scaffold implantation regenerated myocardium, effectively improved cardiac function after myocardial infarction. Therefore, MSCs and PLCL is an excellent biomaterial combination for cardiac tissue engineering.
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