Background: Myocardial bridging is a common cause of non-atherosclerotic ischemic heart disease. The aims of this study were to evaluate the clinical significance of myocardial bridging in coronary angiography (CAG) and 64-row multi-detector computed tomography (MDCT) coronary angiography. Methods: 1023 patients were performed MDCT for the evaluation of atypical chest pain or symptoms suggestive of coronary artery disease. 116 patients (11.3 %) had myocardial bridging in MDCT. Among them, 33 patients were underwent conventional coronary angiography. Correlation was made between the clinical diagnosis according to the patient���s history and the imaging diagnosis according to MDCT or CAG. Results: Among the 33 patients, who were detected myocardial bridge by MDCT, 12 patients (36.4%) were correlation with clinical symptom. All cases of myocardial bridging were located at the middle third of the left anterior descending coronary artery. Fourteen patients (42%) of myocardial bridging were correlation with conventional CAG. Among them, 3 patients (21.4%) were related with clinical symptom. Superficial bridging was identified in 26 patients and deep bridging in 7 patients by MDCT. In superficial bridge patients, 8 patients (30.8%) might relate with clinical symptom. Four patients (57.1%) who had deep bridge in 7 patients were correlation with clinical symptom. Conclusions: Our study shows that MDCT is a more reliable and sensitive diagnostic tool for diagnosing myocardial bridging than conventional CAG. The incidence of myocardial bridging was higher than most of angiographic studies in the literature. However, to verify the correlation between the clinical symptom and the existence of myocardial bridging in MDCT, the larger study should be needed.