Background and Objective; Recent studies suggest that variants of the genes encoding paroxygenase-1 (PON1) and arachidonate 5-lipoxygenase-activating protein (ALOX5AP), which are known to be essential regulators of LDL-oxidation and leukotrien A4 synthesis respectively, are susceptibility factors for myocardial infarction (MI) and coronary artery disease (CAD). Based on the different pathogeneses involved in MI and CAD, the pattern of PON-1 and ALOX5AP polymorphisms are potentially different. We assessed the association of PON1 and ALOX5AP single-nucleotide polymorphisms in the patients with acute ST elevation MI (STEMI) underwent primary PCI, angiographically documented CAD and normal coronary. Methods; Ninety one STEMI patients underwent primary PCI, seventy four CAD patients and fifteen patients with normal coronary angiogram (NL) were recruited between 2007 and 2010 from Chungbuk National University Hospital. All were males. Polymorphisms of PON1 198 Q/R and ALOX5AP (SG13S114 T/A) were genotyped by polymerase chain reaction and the restriction enzyme analysis. And the conventional coronary risk factors were assessed. Results: There was no difference in age at three group (STEMI, N=91, 59.0±13.4 year-old vs CAD, N=74, 61.6±10.9 year-old vs NL, N=15, 63.3±9.2 year-old, P=NS, respectively). Allele patterns for PON1 198 Q/R (STEMI, QQ: 49.4, QR: 18.7, RR: 31.9 % vs CAD, QQ: 41.9, QR: 20.2, RR: 37.9 %) and for ALOX5AP (SG13S114 T/A) (STEMI, TT: 31.9, TA: 43.9, AA: 24.2 % vs CAD, TT: 43.2, TA: 35.1, AA: 21.6 %) in STEMI and CAD were similar. In STEMI and CAD, R allele frequency and A allele frequency are higher than those of NL (R allele; STEMI: 0.41 vs CAD: 0.48 vs NL: 0.37, A allele; STEMI: 0.46 vs CAD: 0.39 vs NL: 0.2). Interestingly, R and A homozygotes, RR and AA, were distributed in young adults (< 55 year-old) compared to old adults (> 55 year-old) in CAD group (RR+AA: < 55 year-old vs > 55 year-old, 31.6 vs 0.0 %). In SETMI group, these RR and AA homozygotes were evenly distributed. Conclusion: These findings suggest that genetic variants of paroxygenase-1 198 Q/R and ALOX5AP (SG13S114 T/A) are associated with an increased risk for CAD in young adults.
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